Aging Methods and Protocols (Methods in Molecular Medicine) by Yvonne A. Barnett

By Yvonne A. Barnett

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Coverslips 1. Place coverslips in a clean, glass rack using forceps. 2. Lower the rack containing the coverslips into a solution of chloroform/95% ethanol (1:1) and allow to soak for 30 min. 3. Rinse the coverslips with deionized water. 4. Submerge the coverslips in a 95:5 solution of concentrated sulfuric acid (95%)/ concentrated nitric acid (70%), previously prepared in a fume hood and allowed to cool to room temperature. Soak the coverslips in this solution for 30 min. 5. Rinse the coverslips thoroughly in deionized water.

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1983) Age-dependent production of a competent factor by human fibroblasts. J. Cell. Physiol. 114, 61–67. 121. Kondo, H. and Yonezawa, Y. (1995) Fetal-adult phenotype transition, in terms of the serum dependency and growth factor requirements, of human skin fibroblast migration. Exp. Cell Res. 220, 501–504. 122. , Reagan, J. , Yan, L. , and Ames, B. N. (1995) Oxidative DNA damage and senescence of human diploid fibroblasts. Proc. Natl. Acad. Sci. USA 92, 4337–4341. 123. Venable, M. , Lee, J. , Smyth, M.

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