Analytical Techniques in Biochemistry and Molecular Biology by Rajan Katoch

By Rajan Katoch

Advances in biochemistry now let us regulate dwelling structures in ways in which have been undreamt of a decade in the past. This quantity courses researchers and scholars throughout the complete spectrum of experimental protocols utilized in biochemistry, plant biology and biotechnology.

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Indicator Indicator is a substance that indicates the physico-chemical status of a reaction. They may be internal, external or self indicators. They are mostly organic compounds of high molecular weight. When dissolved in water or any suitable solvent, they behave either as a weak acid or weak base. Basic indicator possess a coloured cation and acidic indicator possess coloured anion. The internal structural rearrangement is responsible for colour change. 2 Weak acid and weak base green 5. Preparation of indicators Prepare indicators by dissolving requisite quantities of indicators in the solvents as shown below after thorough mixing followed by filtration through Whatman No.

3. 2% Boric acid (w/v): Dissolve the calculated amount of boric acid in distilled water by constant stirring and slight warming, as boric acid is sparingly soluble at room temperature. g. To prepare 1,000 mL, take 20 g boric acid and approximately 980 mL of distilled water. It is required for preparing Tashiro’s indicator as described earlier. 4. 40% NaOH (w/v): Dissolve weighed quantity of crude or commercial sodium hydroxide flakes in ordinary tap water by constant stirring. Store after cooling.

This can be explained as under: The atomic weight of fluoride is 19; hence, a 10À3 M solution of a fluoride salt contains 19 ppm fluoride. 1 M solution is, therefore, equivalent to 19,000 ppm fluoride. The atomic weight of Ca ¼ 40; hence, a 10À3 M solution of CaCO3 contains 40 ppm calcium and 10À2 M solution is equivalent to 400 ppm of calcium. Or Atomic weight of Ca ¼ 0 and 1 M ¼ 40 g or 40,000 mg ¼ 40,000 ppm; hence, 10À2 M solution is equivalent to 40,000  10À2 ppm ¼ 400 ppm. 2. 3. G. 17 1  50 mL  10 m 5 g  1 mL Preparation of Standard Solution of Acids and Bases The preparation of standard acid solution, viz.

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